Keywords
Abstract
Objective: This study aimed to determine gender-wise variation in dengue positivity rate and predilection of children towards dengue severity.
Study Design: A Analytical cross-sectional study was conducted.
Place and duration of study: A cross-sectional analytical study was done among 847 children who came to dengue OPD of Holy Family Hospital Rawalpindi during October-November 2024.
Material and Methods: A cross-sectional analytical study was done among 847 children who came to dengue OPD of Holy Family Hospital Rawalpindi during October-November 2024. Pediatric cases tested positive on NS1 and IgM testig were included in the study through consecutive non-probability sampling. Data was analyzed by means of SPSS version 27.0. Descriptive statistics were applied. Cases were segregated with respect to different dengue clinical syndromes. Gender-wise difference in dengue positivity rate was determined by applying chi-square test and calculating Odd’s ratio along with 95% Confidence Interval (CI). P < 0.05 was taken as significant.
Results: Out of 847 children examined and investigated in dengue OPD, 124 were dengue positive. There were 69.3% were males 30.7% were females among dengue positive children. 71% of them had Dengue Fever (DF) while 25% and 4% were diagnosed with Dengue Hemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS) respectively. Overall dengue positivity rate was 14.6% while rate among males and females was 17.1% and 11% respectively. Gender-stratified dengue positivity results revealed statistically significant difference (P = 0.013) with Odd’s ratio of 1.67 (95% CI= 1.11 – 2.51)
Conclusion: Male children exhibited significantly higher dengue positivity rate than female children of Rawalpindi.
Keywords: dengue positivity rate, tertiary care hospital, dengue fever, dengue hemorrhagic
References
1. Paz-Bailey G, Adams LE, Deen J, Anderson KB, Katzelnick LC. Dengue. Lancet. 2024 Feb 17;403(10427):667-682. https://doi.org/10.1016/s0140-6736(23)02576-x.
2. World Health Organization. Dengue and Severe Dengue. 2024. Available at: https://www.who.int/health-topics/dengue-and-severe-dengue#tab=tab_2.
3. Deng J, Zhang H, Wang Y, Liu Q, Du M, Yan W, et al. Global, regional, and national burden of dengue infection in children and adolescents: an analysis of the Global Burden of Disease Study 2021. eClinicalMedicine. 2024 Nov 19; 78: 102943. https://doi.org/10.1016/j.eclinm.2024.102943.
4. Lin H, Tun MMN, Zin YMW, Myint KT, Khine WK, New KM, et al. Comparison of clinical and virological features in pediatric and adult dengue cases at Insein General Hospital during Myanmar’s 2022 dengue season. Trop Med Health 2025; 53: 13. https://doi.org/10.1186/s41182-025-00688-7.
5. Ghosh K, Tony SR, Islam K, Mobarak R, Alam MJ, Kamruzzaman M, et al. Epidemiology of pediatric dengue virus infection, Scenario from a tertiary level hospital in Bangladesh. J Infect Public Health. 2025 Apr; 18(4): 102684. https://doi.org/10.1016/j.jiph.2025.102684.
6. Umair M, Rehman Z, Haider SA, Ali Q, Jamal Z, Ammar M, et al. Genomic Characterization of Dengue Virus Outbreak in 2022 from Pakistan. Vaccines 2023; 11(1): 163. https://doi.org/10.3390/vaccines11010163.
7. Saha P, Saha P, Gupta SD, Chattopadhyay W, Das S. Spreading of dengue virus infection cases in Malda district of West Bengal, India during COVID-19 Pandemic situation (2020-2022): A cross-sectional study. J Res Clin Med 2024; 12: 37. https://doi.org/10.34172/jrcm.34496.
8. Sharma P, Malhotra B, Sharma H, Bhomia N, Deeba F, Kuldeep A, et al. Trends in dengue virus positivity & serotyping in Rajasthan. Indian J Med Res 2024; 160(5): 479-488. https://doi.org/10.25259/ijmr_1343_23.
9. Gul M, Latifullah, Irshadullah, Asghar M, Rehman N, Mahnoor, et al. Clinico-Pathological Spectrum of Dengue Fever among Children in District Peshawar. J Saidu Med Coll. 2025;15(2):255-260. https://doi.org/10.52206/jsmc.2025.15.2.1015.
10. Ministry of National Health Services Regulations & Coordination. Advisory for the prevention and control of dengue fever. March 2025. Available at: http://www.nhsrc.gov.pk.
11. UNICEF South Asia. Dengue: How to keep children safe. September 2023. Available at: https://www.unicef.org/rosa/stories/dengue-how-keep-children-safe#:~:text=Young%20children%20and%20particularly%20infants,in%20children%20can%20be%20avoided.
12. DEAG Guidelines, Government of Punjab 2024. Available at: https://deag.punjab.gov.pk/download_links.
13. World Health Organization. A toolkit for national burden estimation. 2018. https://iris.who.int/server/api/core/bitstreams/215d47b2-fc74-4b93-a559-9f56f1d3f7ac/content.
14. Anker M, Arima Y. Male-female differences in the number of reported incident dengue fever cases in six Asian countries. Western Pac Surveill Response J 2011 Jun 30; 2(2): 17-23. https://doi.org/10.5365/wpsar.2011.2.1.002.
15. Zohra T, Din M, Ikram A, Bashir A, Jahangir H, Baloch IS, et al. Demographic and clinical features of dengue fever infection in Pakistan: a cross-sectional epidemiological study. Trop Dis Travel Med Vaccines. 2024 Apr 5;10(1):11. https://doi.org/10.1186/s40794-024-00221-4.
16. Baiz MMI, Rauf HA, Ahmed A, Fatima A. Clinical course and outcome of patients with dengue fever, dengue hemorrhagic fever and dengue shock syndrome in a tertiary care hospital in recent endemic 2022. Pak Postgrad Med J 2024; 35(1): 16-21. http://ppmj.org.pk/index.php/ppmj/article/view/651.
17. Anker M, Arima Y. Male-female differences in the number of reported incident dengue fever cases in six Asian countries. Western Pac Surveill Response J. 2011 Jun 30;2(2):17-23. https://doi.org/10.5365/WPSAR.2011.2.1.002.
18. Kaplan JE, Eliason DA, Moore M, Sather GE, Schonberger LB, Cabrera-Coello L, et al. Epidemiologic investigations of dengue infection in Mexico, 1980. Am J Epidemiol 1983 Mar; 117(3): 335-343. https://doi.org/10.1093/oxfordjournals.aje.a113546.
19. Mishra S, Ramanathan R, Agarwalla SK. Clinical profile of dengue fever in Children: A study from Southern Odisha, India. Scientifica (Cairo) 2016; 2016: 6391594. https://doi.org/10.1155/2016/6391594.
20. Shah GS, Islam S, Das BK. Clinical and laboratory profile of dengue infection in children. Kathmandu Univ Med J (KUMJ). 2006 Jan-Mar;4(1):40-43. PMID: 18603866. https://pubmed.ncbi.nlm.nih.gov/18603866/.
21. CDC. Clinical Features of Dengue. May 15, 2025. https://www.cdc.gov/dengue/hcp/clinical-signs/index.html.
22. Khan MAS, Al Mosabbir A, Raheem E, Ahmed A, Rouf RR, Hasan M, et al. Clinical spectrum and predictors of severity of dengue among children in 2019 outbreak: a multicenter hospital-based study in Bangladesh. BMC Pediatr 2021 Oct 29; 21(1): 478. https://doi.org/10.1186/s12887-021-02947-y.
23. Gupta S, Teachey DT, Chen Z, Rabin KR, Dunsmore KP, Larsen EC, et al. Sex-based disparities in outcome in pediatric acute lymphoblastic leukemia: a Children's Oncology Group report. Cancer 2022 May 1; 128(9): 1863-1870. https://doi.org/10.1002/cncr.34150.
24. Bodinayake CK, Nagahawatte AD, Devasiri V, Dahanayake NJ, Wijayaratne GB, Weerasinghe NP, et al. Outcomes among children and adults at risk of severe dengue in Sri Lanka: Opportunity for outpatient case management in countries with high disease burden. PLoS Negl Trop Dis. 2021 Dec 28;15(12): e0010091. https://doi.org/10.1371/journal.pntd.0010091.
25. Jahangir S, Zanjani SM, Zanjani SMS, Wahid S, Amin M, Nawaz S. Clinical Profile and Outcome of Dengue Fever in Pediatric Patients: A Hospital-Based Study. Pakistan Journal of Medical & Health Sciences 2023; 17(12): 724. https://doi.org/10.53350/pjmhs020231712724.
26. Haider N, Hasan MN, Onyango J, Billah M, Khan S, Papakonstantinou D, et al. Global dengue epidemic worsens with record 14 million cases and 9000 deaths reported in 2024. Int J Inf Diseases Sep 2025; 158: 107940. https://doi.org/10.53350/pjmhs020231712724.
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